Category Research project
  • Expositionsschätzung

Validation of an impedance-based test for eye damage which allows the identification of all GHS categories

Project status
Completed
Project start
Jun 2020
Project end
Jun 2023
Acronym
ImAI
Department
Experimentelle Toxikologie und ZEBET

Description and Objective

The aim of the project was to further develop and (pre)validate a test method for assessing the risk of chemicals to the human eye, which detects all categories according to the globally harmonized system of classification and labeling of chemicals (GHS). For this purpose, an established test method, which consists of a combination of an in vitro cornea model and non-invasive impedance spectroscopy as an analysis method, should be further developed and (pre)validated. The BfRshort forGerman Federal Institute for Risk Assessment was involved in WP1 “Study design and test substance selection”, WP4 “Parameterization of the test procedure and method transfer” and WP5 “Validation of impedance-based irritation testing” of the joint project.

Result

WP1 was successfully completed as planned in collaboration with FhG, GU and Clariant with the determination of the test set for the validation study. A set of 30 substances was made available to carry out the validation study within WP5. In WP4, the BfRshort forGerman Federal Institute for Risk Assessment contributed to the further development of the test protocol to improve prediction as planned. As a result, an optimized SOP and two suitable prediction models were developed, which formed the basis for the validation study in WP5. During the project period, two runs of the validation study in WP5 were undertaken. The second run was necessary because the variability of the models in the first run was too high and classification of the test substances was therefore not possible. Due to time constraints, only liquids could be tested in the second run, i.e. the validation of the method for solids is still pending. To better differentiate between Cat1 and Cat2 substances, the prediction model should be further optimized. Contrary to the original plan, the validation of the method could not be completely finalized; further work will be carried out as part of ImAi-2.
Type of project

Third-party funded project

Research focus

Alternativmethoden zum Tierversuch

Organisational units and partners

Lead specialist group: Toxikologische Bewertungsstrategien (93) External partner: Institut für Silicatforschung, Goethe-Universität Frankfurt am Main, Clariant Produkte GmbH, Courage + Khazaka Electronic GmbH

Funding body and grant number

Bundesministerium für Bildung und Forschung
031L0227B

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