Expanding the scope and reaching regulatory acceptance of the impedance-based eye irritation test

The goal of the follow-up project lmAi-2 is to extend the applicability domain of the ImAI test method and to submit it to the OECD as a test guideline for the assessment of eye damaging substances. In order to extend the applicability domain, surfactants, detergents and poorly soluble substances in particular are to be tested, which have proved difficult in the methods available to date for determining eye damaging properties. Furthermore, it is planned to identify and test relevant mixtures and formulations and to expand the scope of application accordingly. In addition, the transferability to commercially available cornea models already implemented in an OECD guideline will be demonstrated to ensure the worldwide availability of the method.