Category Research project
  • Toxikologie

Comparative metabolism of 2-monochloropropane-1,3-diol (2-MCPD) and 3-monochloropropane-1,2-diol (3-MCPD) in rats and humans

Project status
In Progress
Project start
Mar 2022
Project end
Aug 2026
Acronym
Metabolismus von chlorierten Propandiolen
Department
Lebens- und Futtermittelsicherheit in der Nahrungskette

Description and Objective

Fatty acid esters of 2-monochloropropane-1,3-diol (2-MCPD) and 3-monochloropropane-1,2-diol (3-MCPD) are common heat-induced food contaminants, which are hydrolyzed efficiently in the gastrointestinal tract to release 2/3-MCPD. The concepts of the molecular mechanisms explaining the toxicity and carcinogenicity of 2/3-MCPD in rats are limited. A better understanding depends on the available information on the metabolism. The current proposal aims at the comparative elucidation of the metabolism of 2/3-MCPD in rats and humans. The rats will receive single oral doses of 2- or 3-MCPD and also of [13C3]2-MCPD or [13C3]3-MCPD. The urinary metabolites will be separated and characterized by mass spectrometry (MS) and 13C-nuclear magnetic resonance (NMR) spectroscopy. The data on the compounds will be the basis for the characterization of 2/3-MCPD metabolites excreted in human urine samples. The information of the 2/3-MCPD metabolism is a good basis for the choice of human biomarkers of exposureExposureTo glossary. We will develop analytical techniques for the quantification of metabolites of 2/3-MCPD in 24-h urine, which meet the requirements on sensitivity and specificity. The selected biomarkers will be used to estimate the external 2/3-MCPD exposure of study participants with distinct dietary habits (omnivores, vegans and raw food eaters). In summary, the data provided on 2/3-MCPD metabolism in rats and in humans will help to understand the toxic effects in rats and, possibly, the relevance for humans, and will support determining the human exposure in individuals and future recommendations on risk management. Specific Aims 1. Identification of individual metabolites of 2- and 3-MCPD in rats. 2. Identification of individual metabolites of 2- and 3-MCPD in humans following uptake of contaminated hazel nut oil. 3. Development of isotope-dilution LC-MS/MS methods for the quantification of the main human metabolites of 2- and 3-MCPD and testing their applicability as biomarkers of exposur
Type of project

Third-party funded project

Research focus

Moderne Methoden in der Toxikologie / Expositionsabschätzung und Bewertung biologischer Risiken

Organisational units and partners

Lead specialist group: Ernährungsrisiken und Biomarker (84)
Contact persons: Dr. Bernhard Monien
Involved BfR partners: Sicherheit in der Nahrungskette
External partner: Fachbereich 1.1 - Anorganische Spurenanalytik, SGS Germany GmbH

Funding body and grant number

Deutsche Forschungsgemeinschaft e.V.
MO 2520/2-1

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